FIGURE

Fig. 3

ID

ZDB-FIG-141105-2

Publication

Ichimura et al., 2013 - Podocalyxin regulates pronephric glomerular development in zebrafish

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Fig. 3

Localization of Podocalyxin in the developing pronephric glomerulus. Double immunofluorescence labeling for ZO1 (green) and Podocalyxin (red) was performed to examine the localization of Podocalyxin in zebrafish at 34 hpf (A-A′′), 40 hpf (B-B′′), 2 dpf (C-C′′), 3 dpf (D-D′′), and 4 dpf (E-E′′). At 34 hpf, immunoreactivity for ZO1 is found at the tight junctions between the primitive columnar podocytes (arrowheads in A, A′′). In addition, Podocalyxin is localized at the apical membrane, which is recognized as the luminal surface membrane above the tight junctions (A′, A′′). At 40 hpf, the Podocalyxin-localized apical membrane is expanded (B-B′′). After 2 dpf, immunoreactivity for ZO1 is found along the glomerular wall (C, D, and E), which indicates that interdigitation between the podocytes has become extensively. The Podocalyxin signal is found along the entire surface of the podocyte cell bodies (C′-E′, C′′-E′′). (A′′-E′′) show the merged images. N, notochord; arrows, tight junctions in the intestinal epithelium. Bar scale, 10 μm.

Expression Data

Genes:	podxl tjp1a
Antibody:	Ab1-podxl
Fish:	AB
Anatomical Terms:	intestinal epithelium bicellular tight junction pronephric podocyte apical plasma membrane pronephric podocyte bicellular tight junction pronephric podocyte plasma membrane
Stage Range:	Prim-15 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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