Fig. 2

Aldh7a1 is important for optic fissure closure.

(A–D) Injection of Aldh7a1 morpholino results in failure of optic fissure to close at 28 hpf and sustained at 5 dpf. (A) Eye of control morpholino (MO) injected embryo at 28 hpf, (B) Eye of aldh7a1 morphant at 28 hpf; (C) Ventral view of eye in control MO embryo at 5 dpf, (D) Ventral view of eye in embryos injected with 7.5 ng Aldh7a1 MO at 5 dpf, black bars indicate edges of optic fissures; (E) Bar graph demonstrate distribution of eye phenotypes 0, I, II, and III at 28 hpf following 7.5 ng Aldh7a1 MO injection followed by partial rescue of phenotype upon co-injection of two doses of aldh7a1 mRNA. All control MO injected embryos displayed “0” phenotype. (F) zn-5 staining of control MO injected embryos at 48 hpf compared to (G) aldh7a1 morphant which displays optic nerve hypoplasia, arrow-heads indicate optic nerve. Scale bar: 65 μm in A,B; 125 μm in C,D; 75 μm in F,G.