FIGURE

Fig. 2

ID

ZDB-FIG-140909-29

Publication

Apschner et al., 2014 - Pathological mineralization in a zebrafish enpp1 mutant exhibits features of Generalized Arterial Calcification of Infancy (GACI) and Pseudoxanthoma Elasticum (PXE)

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Fig. 2

Soft tissue calcifications in dgf mutants probably represent passive calcium depositions. collagen10a1:YFP (col10) transgene in sibling (A) and mutant (B). Note that no expression of collagen10a1 was detected at sites of ectopic mineralization (Alizarin Red) at the heart (B, blue arrow). osteocalcin:GFP (osc) combined with Alizarin staining in siblings (C) and dgf mutants (D), no ectopic expression of osc was observed to colocalize with ectopic mineralization in the heart region and pectoral fin (D, blue arrows). Calcein staining marks calcifications in collagen2a1a:mCherry (col2) transgenic line in wild-type siblings (E) and dgf mutants (F). The dgf mutant shows ectopic calcifications in the cranium (F, white arrows), however no ectopic expression of collagen2a1a was observed. Alizarin staining and collagen10a1:YFP transgene expression in the axial skeleton of a sibling (G) and dgf embryo (H), ectopic mineralization of the notochord sheet occurs independently of collagen10a1 expression (white arrows, H). In situ hybridization for spp1 (blue) in siblings (I,K) and dgf mutants (J,L). Note upregulation of spp1 in mutant bone elements. Further ectopic expression occurs at loci that are frequently affected by ectopic mineralization in mutants (arrowheads in J,L; compare with supplementary material Fig. S1H). cl, cleithrum; ps, parasphenoid; sib, sibling.

Expression Data

Genes:	Citrine EGFP mCherry spp1
Fish:	WT enpp1^{hu4581/hu4581} hu4008Tg hu5910Tg hu7050Tg enpp1^{hu4581/hu4581}; hu4008Tg enpp1^{hu4581/hu4581}; hu5910Tg enpp1^{hu4581/hu4581}; hu7050Tg
Anatomical Terms:	cleithrum cranium extension integument heart parasphenoid vertebra
Stage Range:	Day 4 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	enpp1^{hu4581/hu4581} enpp1^{hu4581/hu4581}; hu4008Tg enpp1^{hu4581/hu4581}; hu5910Tg enpp1^{hu4581/hu4581}; hu7050Tg
Observed In:	chondrocyte differentiation cranium cranium osteoclast heart integument osteoclast notochord osteoblast differentiation
Stage Range:	Day 4 to Days 7-13

Phenotype Detail

Acknowledgments

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