FIGURE

Fig. 7

ID

ZDB-FIG-140903-33

Publication

Heijnen et al., 2014 - Ribosomal Protein Mutations Induce Autophagy through S6 Kinase Inhibition of the Insulin Pathway

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

S6 kinase phosphorylation and autophagy is induced by reactive oxygen species (ROS).

(A) Confocal microscopy analysis of GFP-LC3 HEK cells transfected with siScr or siRPS19 and either untreated or treated with 10 mM Trolox overnight. Size bars = 10 μM. (B) Quantification of the average number of GFP-LC3 puncta per cell in (A). (C) Western blot analysis of phosphorylated S6 kinase expression in GFP-LC3 HEK transfected with siScr or siRPS19 and either untreated or treated with 10 mM Trolox overnight. (D) Western blot analysis of phosphorylated S6 kinase and phosphorylated AKT substrate expression in 2 dpf zebrafish embryos untreated or treated with 10 mM Trolox overnight. (E) Survival rates of embryos treated overnight with 10 mM Trolox. The results are the compilation of three independent experiments with N = 25 embryos representing each mutation. The statistics shown are compared to wild type. (F) Analysis of p53 stabilization in 2 dpf wild type, rpS7-deficient embryos, p53^M214K/M214K mutant embryos, or double mutants (rpS7;p53) by western blotting. (G) Analysis of p53 stabilization by western blotting of zebrafish mutants treated overnight with 100 μM Trolox.

Expression Data

Gene:	tp53
Antibody:	Ab6-rps6k
Fish:	WT rps3a^{hi1290Tg/hi1290Tg} rps7^{hi1034bTg/hi1034bTg} tp53^zdf1/zdf1 tp53^zdf1/zdf1; rps7^{hi1034bTg/hi1034bTg}
Condition:	chemical treatment: Trolox
Anatomical Term:	whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT rpl11^{hi3820bTg/hi3820bTg} rps3a^{hi1290Tg/hi1290Tg} rps7^{hi1034bTg/hi1034bTg}
Condition:	chemical treatment: Trolox
Observed In:	whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.