FIGURE

Fig. 4

ID

ZDB-FIG-140822-61

Publication

Rost et al., 2014 - Hyaluronic acid receptor Stabilin-2 regulates Erk phosphorylation and arterial--venous differentiation in zebrafish

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Fig. 4

Stab2 knockdown affects Erk phosphorylation.

(A–D) Phosphorylated Erk expression is observed in the DA of uninjected control embryos by whole mount immunostaining (A,B), while a majority of Stab2 morphants display a reduction in arterial P-Erk expression (C,D). (E) Percentages of embryos displaying normal or decreased arterial P-Erk expression. Arrows indicate P-Erk expression in the DA (A,C). Experiments performed in a Tg(fli1a:GFP) line. Green staining: GFP, Blue Staining: DAPI, Red staining: P-Erk. Lateral view, anterior is to the left. All embryos are at the 20-somite stage. Morphants were injected with a cocktail containing Stab2 MO1, MO2 and p53 MO.

Expression Data

Genes:	flt4 mrc1a stab1 stab2
Antibody:	Ab7-mapk
Fish:	WT WT + MO1-stab2 + MO4-stab2 + MO4-tp53
Knockdown Reagents:	MO1-stab2 MO4-stab2 MO4-tp53
Anatomical Terms:	dorsal aorta posterior cardinal vein vascular cord
Stage Range:	20-25 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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