Fig. S2

Only high UHRF1 expression leads to a reduced liver size and increased larval death. (Related to Figure 2)

(A) Cy-3 streptavidin stained the entire liver and then the area of the left liver lobe of at least 5 fish from each UHRF1-GFP transgenic line and from control fish on 3-5 dpf was measured. Liver size was only significantly reduced in UHRF1-GFP High fish on 5 dpf as calculated by a Student’s T-test. (B) Standard length of nlsmCherry and UHRF1-GFP High fish on 5 and 10 dpf. (C) Three clutches of each of the UHRF1 over expressing lines (High, Medium and Low) and control fish ((Tg(fabp10:dsRed) or Tg(fabp10:nls-mCherry)) were monitored for survival from 5-500 dpf. A significant decrease in survival by log-rank test was found only for UHRF1-GFP High and UHRF1-GFP Medium lines. (D) UHRF1-GFP High embryos were injected with mRNA encoding zebrafish Dnmt1 or mannose phosphate isomerase as a control (Chu et al., 2013) prior to the 8 cell stage. On 5 dpf, fish were fixed and stained with Cy3-SA to label the liver. The area of theleft liver lobe was calculated for at least 10 fish per condition. Uninjected Tg(fabp10:nls-mCherry) and UHRF1-GFP High larvae were also measured as controls. Percent of liver size was normalized to the median liver size in control, uninjected larvae and indicated on the graph. Boxes represent 75th and 25th percentile, bars indicate lowest and highest values. Error bars are SD.