Fig. 2

UHRF1-Induced Hypomethylation Reduces Liver Size

(A) Individual larvae were imaged daily from 3–10 dpf.

(B) Five dpf UHRF1-GFP High larvae display a range of liver sizes scored as ‘‘normal’’ or ‘‘small’’.

(C) Three clutches were scored according to criteria in (B); n, number of larvae. Fisher’s exact test was used to determine p value.

(D) The area of the left liver lobe was measured in 5 dpf fish from two clutches. Boxes represent 75th and 25th percentile, horizontal line is the median, and whiskers mark lowest and highest values. Student’s t test was used to determine p value.

(E) UHRF1-GFP High larvae were sorted by liver size on 5 dpf and tracked daily for survival to 20 dpf. Data are pooled from three clutches.

(F) UHRF1-GFP High and control larvae were treated with 50 mM 5-Aza from 2.5–5 dpf and scored for liver size in six clutches. Fisher’s exact test was used to determine p values.

(G) UHRF1-GFP High embryos were injected with mRNA encoding dnmt1 or Mpi before 1 hpf. The percent of fish with a normal liver size was scored at 5 dpf in six clutches.