Fig. 4
UHRF1 Is an Oncogene (A) Atypical cells, dysplastic foci (outlined), and HCC are apparent in hematoxylin and eosinstained UHRF1-GFP High livers. BD, bile duct; MF, mitotic figure. (B) Incidence of normal and atypical hepatocytes, dysplastic foci, and cancer in the liver of UHRF1- GFP High fish on WT or tp53+/- background. (C) NIH 3T3 cell growth in soft agar is enhanced when UHRF1 overexpression is combined with RAS (n = 3). The p value was calculated by Student’s t test, and error bars represent the SD. (D) Hepatic SA-b-gal-staining patterns in UHRF1- GFP High larvae change as fish age. Images of 8 dpf larvae illustrate the SA-b-gal-staining patterns that were scored in the time course shown in the graph. A significant increase in the number of UHRF1-GFP High fish with intense or punctate SA-b-gal compared to controls at all time points (p < 0.01 by Fisher’s exact test) except at 4 dpf; n.s., not significant. (E) BrdU incorporation in the liver on 11 dpf is five times higher in UHRF1-GFP High fish than in controls. Total number of cells counted is indicated with n = number of clutches assessed. Fisher’s exact test was used to calculate p value. |
| Genes: | |
|---|---|
| Fish: | |
| Anatomical Term: | |
| Stage: | Days 7-13 |
| Fish: | |
|---|---|
| Observed In: | |
| Stage Range: | Day 5 to Days 14-20 |
Reprinted from Cancer Cell, 25(2), Mudbhary, R., Hoshida, Y., Chernyavskaya, Y., Jacob, V., Villanueva, A., Fiel, M.I., Chen, X., Kojima, K., Thung, S., Bronson, R.T., Lachenmayer, A., Revill, K., Alsinet, C., Sachidanandam, R., Desai, A., SenBanerjee, S., Ukomadu, C., Llovet, J.M., and Sadler, K.C., UHRF1 overexpression drives DNA hypomethylation and hepatocellular carcinoma, 196-209, Copyright (2014) with permission from Elsevier. Full text @ Cancer Cell