FIGURE

Fig. 7

ID

ZDB-FIG-140527-29

Publication

Rydeen et al., 2014 - Cyp26 enzymes are required to balance the cardiac and vascular lineages within the anterior lateral plate mesoderm

Other Figures

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Fig. 7

Cyp26-deficient embryos have decreased anterior endothelial cell number. (A,B) Optical sections of uninjected sibling and Cyp26-deficient Tg(kdrl:nEGFP) embryos show that Cyp26-deficient embryos have mispatterned cranial vasculature. (C,D) Representative immunostained Tg(kdrl:nEGFP) embryos for counting the cranial endothelial and endocardial cells (green). Somites and myocardial cells are indicated in red. (E) Endothelial and endocardial cell counts at 28hpf. WT, n=10; Cyp26-deficient, n=11. All images are lateral views with anterior towards the right. Arrowheads in A and B indicate primordial midbrain channel and the anterior and middle cerebral veins. Arrows in A-D indicate the somite boundary. Significant differences compared with controls are indicated (*P<0.05). Error bars indicate s.d.

Expression Data

Gene:	EGFP
Antibody:	Ab-MF20
Fish:	ubs1Tg ubs1Tg + MO1-cyp26c1 + MO4-cyp26a1 + MO5-cyp26a1
Knockdown Reagents:	MO1-cyp26c1 MO4-cyp26a1 MO5-cyp26a1
Anatomical Terms:	cranial vasculature myocardium somite vasculature
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ubs1Tg + MO1-cyp26c1 + MO4-cyp26a1 + MO5-cyp26a1
Knockdown Reagents:	MO1-cyp26c1 MO4-cyp26a1 MO5-cyp26a1
Observed In:	cranial vasculature endocardium endothelial cell
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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