FIGURE

Fig. 3

ID

ZDB-FIG-140527-13

Publication

Jiang et al., 2014 - Gene-expression analysis of hair cell regeneration in the zebrafish lateral line

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Fig. 3

The Wnt/β-catenin pathway is inactive during the early stages of hair cell regeneration. (A) Still images of a Tg(sqET20;sqET4) larval neuromast in the process of neomycin-induced regeneration. All hair cells, except for two immature hair cells, were killed by neomycin by 1 h after neomycin treatment. Two newly formed hair cells (arrows) start to express GFP at 5 h, and other pairs of hair cells (arrows) appear at 12 and 18 h. (B) In situ hybridization of Wnt/β-catenin pathway genes in 5-dpf untreated (control) larvae and in larvae at different time points after neomycin treatment. Expression of wnt10a is increased at 3 and 5 h. Expression of tcf7l2 (tcf4), fzd7b, and fzd8a is largely decreased at 1 h after neomycin. The Wnt/β-catenin reporter line Tg(Tcf/Lef-miniP:dGFP) demonstrates activation starting at 12 h as shown by dgfp expression, but lef1 is not induced in regenerating neuromasts. (C) Heat map of selected Wnt/β-catenin pathway genes based on RNA-Seq results. Log2 ratios of GFP and GFP⁺ cells at 1, 3, and 5 h after neomycin treatment are color coded (red: up-regulation, blue: down-regulation). Genes that are modulated in GFP cells are neomycin-induced genes.

Expression Data

Genes:	EGFP fzd7b fzd8a GFP lef1 tcf7l2 wnt10a
Fish:	WT kyu1Tg sqet20Et; sqet4Et
Condition:	chemical treatment: pharmaceutical
Anatomical Term:	neuromast
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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