FIGURE

Fig. 3

ID

ZDB-FIG-140506-8

Publication

Dash et al., 2014 - sept7b is essential for pronephric function and development of left-right asymmetry in zebrafish embryogenesis

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Fig. 3

Depletion of sept7b causes defects in the structure of the pronephros. (A–E) Electron micrographs of control and sept7b-morphant kidneys at 4 dpf. (A) Wild-type podocytes show regular foot processes connected by slit diaphragms (arrows). Glomerular basement membrane, GBM. (B,C) Podocytes of a sept7b morphant (TBMO) show foot process effacement (arrowhead) and microvillar-type cell processes (m) on their apical surface. Occasional slit diaphragms (arrow) were observed. (D) A pronephric tubule of the wild-type larva (ctrl) shows regular microvilli (mv) and cilia (c) morphology. (E) A tubule in a sept7b morphant appears dilated and shows sparse and irregular microvilli and scattered cilia. (F,G) The cilia in both control (F) and TBMO morphants (G) show the normal 9+2 structure of microtubules. The outer dynein arms are visible in both the control and sept7b morphant cilia (arrowheads). Scale bars: 1μm (A–C); 10μm (D,E); 140nm (F,G).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-septin15
Knockdown Reagent:	MO1-septin15
Observed In:	pronephric podocyte pronephric podocyte apical part of cell pronephric podocyte cell projection pronephric podocyte slit diaphragm pronephric tubule pronephric tubule microvillus pronephros morphogenesis
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.