FIGURE

Fig. 5

ID

ZDB-FIG-140506-20

Publication

Heim et al., 2014 - Oocyte polarity requires a Bucky ball-dependent feedback amplification loop

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Fig. 5

buc transgenes lacking introns disrupt RNA localization. (A) RT-PCR on ovary lysate cDNA. Transgenic and endogenous transcript expression in ovaries of F1 transgenic females expressing intron-lacking transgenes in buc^p106/+ heterozygotes. (B,C) buc RNA in ovaries of cbuc80+;buc^p106/+ F1 transgenic females (B) resembles WT or (C) is not asymmetrically localized. (D) Quantification of buc RNA expression patterns in stage I oocytes. (E,F) vasa expression in (E) F1 transgenic- and (F) cbuc80 F1 transgenic+ ovaries. (B,E) Blue arrows indicate the Bb. (G) Quantification of vasa expression patterns. (H-J) Relative expression of (H) cbuc80, (I) endogenous buc and (J) vasa in sorted oocytes of F1 or F2 females. (D,G) het, buc^p106/+; mut, buc^p106/p106; wt, buc^+/+.

Expression Data

Genes:	buc ddx4
Fish:	buc^p106re/+ buc^p106re/+; ae7Tg
Anatomical Term:	ovary
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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