ZFIN Image: Heim et al., 2014, Fig. 5

Image

Figure Caption

Fig. 5

buc transgenes lacking introns disrupt RNA localization. (A) RT-PCR on ovary lysate cDNA. Transgenic and endogenous transcript expression in ovaries of F1 transgenic females expressing intron-lacking transgenes in buc^p106/+ heterozygotes. (B,C) buc RNA in ovaries of cbuc80+;buc^p106/+ F1 transgenic females (B) resembles WT or (C) is not asymmetrically localized. (D) Quantification of buc RNA expression patterns in stage I oocytes. (E,F) vasa expression in (E) F1 transgenic- and (F) cbuc80 F1 transgenic+ ovaries. (B,E) Blue arrows indicate the Bb. (G) Quantification of vasa expression patterns. (H-J) Relative expression of (H) cbuc80, (I) endogenous buc and (J) vasa in sorted oocytes of F1 or F2 females. (D,G) het, buc^p106/+; mut, buc^p106/p106; wt, buc^+/+.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development