The no soul embryos are defective in visceral sensory neurons. Anterior is to the left. Lateral views of wild-type (left) and no soul (right) embryos expressing islet-GFP (A-J) or labeled with the monoclonal antibody mAb16A11 (K-L). (A-D) 26 hpf embryos showing that the VIIth ganglia are not generated in the mutant, whereas cranial motor neurons appear normal. (E-H) 48 hpf embryos showing that the VIIth and IXth ganglia are absent while the Xth ganglia are reduced. (I,J) ~3-day old embryos showing persistent defects in visceral sensory neurons of the mutant. (K,L) Anti-Hu antibody labeling of 50 hpf embryos showing that the trigeminal ganglia appear normal, while both epibranchial and lateral line ganglia are defective in the no soul mutant. The identity of the no soul mutant embryos shown in all figures is confirmed by genotyping with closely linked polymorphic markers Z1400 and Z13938. AD, anterior dorsal ganglia; AV, anterior ventral ganglia; M, middle lateral line; mV/mVII/mX, the Vth, VIIth, or Xth motor neurons; ov, otic vesicle; OT, ocular and trochlear motor nuclei; P, posterior lateral line; V, trigeminal ganglion; VII, geniculate ganglion; IX, petrosal ganglion; X, nodose ganglion. Scale bar: 100 μm (A-B, E-F, K-L), 70 μm (C-D, G-J).
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