foo mutants show post-migratory NC cell defects. Right panels, foo/foo homozygote embryos; left panels are same-clutch wild-type siblings. (A,B) dlx2 expression by in situ hybridization in wild-type (A) and foo/foo (B) 28 hpf embryos. Arrows indicate third stream derived third and fourth arch NC cells. Note the apparent loss of the third and fourth arch expression domains in the mutant. (C) hand2 expression by in situ hybridization in wild-type (C) and foo/foo (D) 28 hpf embryos. Arrows indicate third stream derived third and fourth arch NC cells. Note the reduction and disorganization of the third and fourth arch expression domains in the mutant. Also note the normal ventral expression of hand2 in the first and second arches. (E,F) gsc expression by in situ hybridization in wild-type (E) and foo/foo (F) 34 hpf embryos. Arrows in E indicate dorsal gsc expression domains in the first and second arch NC cells flanking either side of pp1. Arrowhead in E indicates gsc expression in the otic vesicle. Arrows in F indicate where gsc expression can no longer be seen in the more dorsal region of expression; more ventral domains remain in foo embryos. Otic vesicle expression is also absent (arrowhead in F).
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