FIGURE

Fig. 3

ID

ZDB-FIG-140306-6

Publication

Reifers et al., 1998 - Fgf8 is mutated in zebrafish acerebellar (ace) mutants and is required for maintenance of midbrain-hindbrain boundary development and somitogenesis

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Fig. 3

Fgf8 expression in early midbrain and anterior hindbrain development. (A-D) Double ISH with Fgf8 (blue) and Krox20 (red, fluorescent) of wild-type embryo at tailbud stage (A,B) and 5 somites (C,D). At tailbud stage, Fgf8 expression extends throughout the anterior hindbrain incl. rhombomere 4 (r4) posteriorly. At 5 somites, expression of Fgf8 is detected at the MHB, in r1, r4 and in ventral r2 (see also Fig. 2H). (E-J) Double ISH with Fgf8 (blue) and Pax2.1 (red, fluorescent) at 90% epiboly (E-H) and 6 somites (I,J). At 90% epiboly, the Fgf8 expression domain is located posterior to the Pax2.1 domain with very little overlap (E,F; higher magnification: G,H), while at 6 somites the Fgf8 domain at the MHB is completely included in the Pax2.1 expression domain (visible as quenching of the fluorescent Pax2.1 signal). Embryos in C-J are flat mounted, A,C,E,G and I show bright field, B,D,F,H and J show fluorescent images of the same embryos.

Expression Data

Genes:	egr2b fgf8a pax2a
Fish:	WT
Anatomical Terms:	hindbrain neural keel anterior region hindbrain neural plate anterior region midbrain hindbrain boundary neural keel midbrain neural plate posterior region midbrain neural rod posterior region presumptive rhombomere 1 presumptive rhombomere 2 ventral region presumptive rhombomere 3 presumptive rhombomere 4 presumptive rhombomere 5
Stage Range:	90%-epiboly to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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