FIGURE

Fig. 1

ID

ZDB-FIG-140214-37

Publication

Valvezan et al., 2014 - Oncogenic mutations in Adenomatous Polyposis Coli (Apc) activate mechanistic Target of Rapamycin Complex 1 (mTORC1)

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Fig. 1

Activation of mTORC1 in apc^mcr/mcr zebrafish. Western blot analysis using antibodies to phosphorylated S6 (p-S6), indicating mTORC1 activity, or total S6 on whole embryo lysates from pooled wild-type and heterozygous apc^mcr/+ zebrafish or homozygous apc^mcr/mcr zebrafish at 3 or 4 dpf. mTORC1 activity was increased in the homozygous apc^mcr/mcr zebrafish. (B) Immunohistochemical staining of sagittal sections from wild-type or homozygous apc^mcr/mcr zebrafish at 3 dpf using antibodies to phosphorylated or total S6 and counterstaining with DAPI. mTORC1 was aberrantly active in multiple mesodermal and endodermal derivatives in the homozygous apc^mcr/mcr zebrafish.

Expression Data

Antibodies:	Ab3-rps6 Ab8-rps6
Fish:	apc^hu745/hu745
Anatomical Terms:	endoderm mesodermal cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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