FIGURE

Fig. S8

ID

ZDB-FIG-140124-25

Publication

Brothers et al., 2013 - NADPH Oxidase-Driven Phagocyte Recruitment Controls Candida albicans Filamentous Growth and Prevents Mortality

Other Figures

All Figure Page

Back to All Figure Page

Fig. S8

In the swimbladder, DPI reduces the neutrophil migration to wounding, but not to C. albicans. (A) Diagram illustrating the injection site (red line) in 4 dpf zebrafish. (B and C) 4 dpf mpx:GFP zebrafish were treated from 1 hour pre-injection to 4 hpi with 100 μM DPI (in DMSO) or vehicle (0.8% DMSO). Fish were injected with 5–20 yeasts of C. albicans CAF2-dTomato in the swimbladder. (B) The C. albicans inoculum was quantified by confocal microscopy within 30 minutes post-infection. (C) Neutrophils were enumerated by confocal microscopy, at the site of infection (SOI) 4 hours post infection. Four independent experiments were performed, with comparable results, and individuals from all four experiments are pooled together. Means and standard error of the mean are represented. N = 33, 30, 62, 46, 67 and 35 for the following six conditions: No injection (DMSO treated and DPI treated); PBS injection (DMSO treated and DPI treated); and Caf2-dTomato injection (DMSO treated and DPI treated), respectively. Statistical tests performed were Mann-Whitney (for panel A) and Kruskall-Wallis (for panel B), n.s. non significant, * p<0.05. (D) Representative images of 4 dpf mpx:GFP zebrafish 4 hpi. Images are composites of maximum projection of the red and green channels (20 slices for no injection and PBS injection and 25 slices for CAF2-dTomato injection) overlaid with a single slice in the DIC channel. Blue outlines in images define the swimbladder site of injection where the neutrophils were counted. Scale bars = 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Pathog.