Fig. 4

Mmp17b and Reck are involved in DRG formation.

A, C and E are 26 hpf WISH embryos for mmp17b (A) in red and reck (C) in green. Panel E is a merge of the two images. Reck and mmp17b are expressed near each other in the trunk at this time point. B, D, and F are 26 hpf WISH embryos for mmp17b (B) in red and crestin (D) in green. Panel F is a merge of the two images. Mmp17b and crestin are co-expressed in the trunk at this time point. G, H, and I are confocal image of three color staining of mmp17b (blue), reck (green), and Flk-GFP (red) in the trunk (G) and plexus region (H) of 26 hpf embryos. Asterisks show that mmp17b and reck are expressed next to each other but not in the same cell. Optical section of panel G shown in panel I shows the proximity of mmp17b and reck expression. Panels J-L are cultured HeLa cells on glass coverslips double stained with MMP17 (J, Green) and RECK (K, Red) antibodies. The overlay of image (L) shows both MMP17 and RECK localizes within the cytoplasm in a punctate manner and near the leading edges of the cell indicated by asterisk (*). Image panel stained with DAPI not shown. Scale bar 10 micron.