Mmp17b is essential for proper neural crest cell migration in vivo
- Authors
- Leigh, N.R., Schupp, M.O., Li, K., Padmanabhan, V., Gastonguay, A., Wang, L., Chun, C.Z., Wilkinson, G.A., and Ramchandran, R.
- ID
- ZDB-PUB-131112-3
- Date
- 2013
- Source
- PLoS One 8(10): e76494 (Journal)
- Registered Authors
- Chun, Chang Zoon, Leigh, Noah, Li, Keguo, Ramchandran, Ramani, Schupp, Marcus
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Body Patterning/genetics
- Cell Movement/genetics*
- Embryonic Development/genetics
- Enzyme Activation
- GPI-Linked Proteins/genetics
- GPI-Linked Proteins/metabolism
- Gene Expression Profiling
- Matrix Metalloproteinase 17/chemistry
- Matrix Metalloproteinase 17/genetics*
- Matrix Metalloproteinase 17/metabolism*
- Molecular Sequence Data
- Neural Crest/cytology*
- Neural Crest/metabolism*
- Organ Specificity/genetics
- Sequence Alignment
- Zebrafish
- PubMed
- 24098510 Full text @ PLoS One
The extracellular matrix plays a critical role in neural crest (NC) cell migration. In this study, we characterize the contribution of the novel GPI-linked matrix metalloproteinase (MMP) zebrafish mmp17b. Mmp17b is expressed post-gastrulation in the developing NC. Morpholino inactivation of mmp17b function, or chemical inhibition of MMP activity results in aberrant NC cell migration with minimal change in NC proliferation or apoptosis. Intriguingly, a GPI anchored protein with metalloproteinase inhibitor properties, Reversion-inducing-Cysteine-rich protein with Kazal motifs (RECK), which has previously been implicated in NC development, is expressed in close apposition to NC cells expressing mmp17b, raising the possibility that these two gene products interact. Consistent with this possibility, embryos silenced for mmp17b show defective development of the dorsal root ganglia (DRG), a crest-derived structure affected in RECK mutant fish sensory deprived (sdp). Taken together, this study has identified the first pair of MMP, and their putative MMP inhibitor RECK that functions together in NC cell migration.