FIGURE

Fig. 5

ID

ZDB-FIG-140107-48

Publication

Reichert et al., 2013 - A BMP regulatory network controls ectodermal cell fate decisions at the neural plate border

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Fig. 5

dlx3b/4b are required for NC induction and formation of the NC BMP signalling domain. (A) sox10 and cvl2 ISH on wild-type embryos (left set of panels) and sox10 and mRFP ISH on Tg(BRE:mRFP) embryos (right set of panels). Embryos were injected with 14 ng control MO or 7 ng each of MOs against dlx3b and dlx4b. Arrows indicate the inner expression domain of cvl2 and mRFP. (B) Analysis of expression domains at the NPB in dlx3b/4b morphants as compared with control MO-injected embryos using DFISH against ncad and cvl2. Arrows indicate the inner cvl2 expression domain. Dorsal anterior view, anterior to top left. (C) ISH on embryos injected with dlx3b/4b or control MOs using probes directed against the PPE markers eya1 and six4.1. Dorsal anterior view, anterior to the top. (D) sox10, mRFP and dlx3b ISH on embryos injected with 4 ng cvl2 MO1 or control MO. Embryos were fixed when they had reached the 5-somite (A-C) or 4-somite (D) stage.

Expression Data

Genes:	bmper cdh2 dlx3b eya1 mRFP six4b sox10
Fish:	WT cj100Tg cj100Tg + MO1-bmper WT + MO5-dlx3b + MO5-dlx4b cj100Tg + MO5-dlx3b + MO5-dlx4b
Knockdown Reagents:	MO1-bmper MO5-dlx3b MO5-dlx4b
Anatomical Terms:	ectoderm neural crest neural plate neurogenic field
Stage Range:	1-4 somites to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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