FIGURE

Fig. 7

ID

ZDB-FIG-131220-5

Publication

Lu et al., 2013 - Liver-Specific Expressions of HBx and src in the p53 Mutant Trigger Hepatocarcinogenesis in Zebrafish

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Fig. 7

Immunohistochemical analyses of phosphorylated erk1/2 (p-erk), phosphorylated akt (p-akt), myc, vegf and jnk1 were performed in liver sections prepared from src-overexpressing wild-type zebrafish (A) or p53 mutant zebrafish (B) that displayed hyperplasia, dysplasia, HCC and sarcomatoid HCC (x 400). GFP-mCherry transgenic fish that were 11 months of age were used as controls and are shown in the first row of each panel. Scale bars: 50 μm. (C) Western blot analysis for the activation of erk and akt in the livers of transgenic fish at different stages of HCC development. After measuring the band intensity using the UVP VisionWorks LS software, the relative density was normalized to β–actin. Ratios of p-erk/erk and p-akt/akt were analyzed, and the data are expressed as relative fold changes for src or src(p53^-) transgenic fish relative to GFP-mC controls.

Expression Data

Antibodies:	Ab1-mapk8 Ab2-myc Ab2-vegf Ab5-akt Ab9-mapk
Fish:	nn1003Tg tp53^zdf1/zdf1; nn1004Tg
Anatomical Term:	hepatocyte
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nn1003Tg tp53^zdf1/zdf1; nn1004Tg
Observed In:	hepatocyte hepatocyte mitotic cell cycle hepatocyte nucleolus hepatocyte nucleus liver hepatic tumor liver spindle cell sarcoma
Stage:	Adult

Phenotype Detail

Acknowledgments

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