FIGURE

Fig. 5

ID

ZDB-FIG-131220-3

Publication

Lu et al., 2013 - Liver-Specific Expressions of HBx and src in the p53 Mutant Trigger Hepatocarcinogenesis in Zebrafish

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Fig. 5

(A) Immunohistochemical analysis of src protein expression in hepatocytes from the GFP-mC and p53 mutant control fish and HBx(p53^-) transgenic fish at 11 months. (B) src IHC results from HBx transgenic fish at 1.5, 7, 9 and 11 months. (C) src IHC results from HBx(p53^-) transgenic fish at 1.5, 7, 9 and 11 months. (D) Immunohistochemical detection of phosphorylated ERK and phosphorylated AKT in 11-month-old Tg(l-fabp:HBx-mCherry;cmcl2:GFP) transgenic fish with hyperplasia and HCC (x 400). Scale bars: 50 μm. (E) Western blot analysis for the activation of erk and akt in the livers of transgenic fish at different stages of HCC development. After measuring the band intensity using the UVP VisionWorks LS software, the relative density was normalized to β–actin. The ratios of p-erk/erk and p-akt/akt were analyzed, and the data are expressed as fold changes of HBx(p53^-) transgenic fish relative to GFP-mC controls.

Expression Data

Antibodies:	Ab5-akt Ab6-src Ab9-mapk
Fish:	tp53^zdf1/zdf1 nn1000Tg nn1001Tg tp53^zdf1/zdf1; nn1002Tg
Anatomical Term:	hepatocyte
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nn1001Tg tp53^zdf1/zdf1; nn1002Tg
Observed In:	hepatocyte hepatocyte mitotic cell cycle hepatocyte nucleolus hepatocyte nucleus liver liver hepatic tumor
Stage:	Adult

Phenotype Detail

Acknowledgments

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