Fig. S4

Expression of gpr126 mRNA at other sites during zebrafish development. (A-C) Flat mounted embryos at 5 somites (A), 14 somites (B) and 23 somites (C). Patchy expression of gpr126 is seen the anterior of the embryo and also in presumed neural crest (A). At 14 somites, weak expression is seen in the presumed developing heart fields, neural crest and intermediate mesoderm (im; precursor of the pronephric ducts) (B). At 23 somites, expression increases in the migrating heart tube (h) and neural crest (nc) (C). (D,G,J) Expression at 24 hpf in dorsal (D), lateral (G) and detailed (J) view, showing expression in the post-otic neural crest (nc), heart tube (h) and olfactory epithelium (nose, n). J shows staining of individual cells within the olfactory epithelium. (E,H,K) 48 hpf embryos in dorsal (E), lateral (H) and detailed (K) view. Expression continues in the nose (arrow, H), but is reduced in the heart and lost in the postotic ganglia. New expression is seen in the ear (ov), pectoral fin (f) and tail fin (not shown), and chondrocytes of the head (arrowheads). K shows expression of gpr126 in the chondrocytes of the pectoral fin. (F,I,L) 72 hpf embryos in dorsal (F), lateral (I) and detailed (L) view. Expression continues in the ear, nose, fins and head chondrocytes (arrowheads and line). Detailed view of gpr12 expression in posterior ceratobranchials is shown in L. By 96 hpf, expression of gpr126 remains primarily in the ear (not shown). Abbreviations: b, basihyal; cb, ceratobranchials; ep, ethmoid plate; ch, ceratohyal; f, pectoral fin; gc, gill cartilages; h, heart; im, intermediate mesoderm; m, Meckel’s cartilage, n, nose (olfactory epithelium); nc, neural crest; ov, otic vesicle. Scale bars: A-I, 100 μm; J-L, 50 μm.