FIGURE

Fig. 5

ID

ZDB-FIG-131212-6

Publication

Janssens et al., 2013 - Matrix metalloproteinase 14 in the zebrafish: an eye on retinal and retinotectal development

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Fig. 5

Mmp14a knockdown results in embryos with microphthalmic eyes.

A-D Analysis of eye size (A, C) and total body length (B, D) reveals a dose-dependent decrease in eye size after knockdown of Mmp14a with the ATG- (A, B) or the splice MO (C, D) at 5 dpf. Body length, however, was only decreased after injection of a supramaximal dose of the Mmp14a-ATG MO (B) (n = 55 from 3 independent experiments). E, F Analysis of eye size (E) and total body length (F) (both in µm), in embryos injected with Mmp14a-ATG MO (Mmp14a^KD), reveals that knockdown of Mmp14a leads to persistent microphthalmic eyes in embryos/larvae from 3 to 10 dpf while the overall body length is unaffected as compared to control fish (n = 75 from 3 independent experiments). Data are represented as mean ± SEM (*p<0.05, Student′s t-test for A-D; multilevel model statistical test (SAS proc mixed) for E-F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO4-mmp14a + MO5-tp53 WT + MO5-mmp14a + MO5-tp53
Knockdown Reagents:	MO4-mmp14a MO5-mmp14a MO5-tp53
Observed In:	eye
Stage:	Day 5

Phenotype Detail

Acknowledgments

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