FIGURE

Fig. S5

ID

ZDB-FIG-131211-18

Publication

Colombo et al., 2013 - Daam1a mediates asymmetric habenular morphogenesis by regulating dendritic and axonal outgrowth

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Fig. S5

Bilateral electroporation of daam1a-MO induces defects in neuropil formation and IPN connectivity. (A-F) Dorsal views of maximum intensity z-stack confocal projections of the habenular region (A-D) and IPN (E,F) in Tg(pou4f1-hsp70l:GFP) embryos at 4.5 dpf, with anterior to the top. The habenular neuropil was immunostained against acetylated α-tubulin (A,B, white), while the soma (C,D) and efferent projections (E,F) of dorsal habenular neurons expressing pou4f1-hsp70l:GFP were detected in vivo (green). Each column corresponds to a different condition of local electroporation: control-MO (left) and daam1a-MO (right). Asterisks in C and D indicate the enlarged cellular domain of the Hb expressing the pou4f1-hsp70l:GFP transgene. Scale bar, 20μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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