Fig. 5

Targeting of zebrafish fbxl10 transcript modulates pre-rRNA expression. (A) Schematic of the un-spliced fbxl10 transcript and the location of the fbxl10 morpholino (black bar). Injection of the morpholino results in an exclusion of exon 2 in the mature fbxl10 transcript and is predicted to induce a nonsense mutation (black arrow). Exclusion of exon 2 in the fbxl10 morphant transcript was confirmed by PCR. (B) Graph of qRT-PCR data measuring the expression of wild-type fbxl10 relative to the expression in Std control morphants at 8 hpf (n=4). (C–D) Representative lateral (C) and dorsal (D) bright-field images of fbxl10 morphants.(E–F) Lateral views of representative P-H3 stained zebrafish morphants at 25 hpf. (G) Quantification of the area occupied by P-H3 positive cells in fbxl10 morphants relative to P-H3 positive cells in Std morphants (n=12–13). (H) Graph of qRT-PCR expression data for 45S pre-rRNA relative in fbxl10 morphants relative to Std morphants (n=4). (I) Graph of qRT-PCR data measuring ink4ab expression relative to Std morphants (n=6).Error bars in all graphs represent SEM. Significance determined by a Student′s two-tailed t-test and significant values are noted p<0.05 (*) and p<0.01 (**).