FIGURE

Fig. 2

ID

ZDB-FIG-131105-17

Publication

Rosenfeld et al., 2013 - Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis

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Fig. 2

Cardiac small heat shock proteins are required for morphogenesis of the heart tube and they interact genetically with gata4. (A) Representative cardiac phenotypes observed at 3 dpf of myl7:gfp embryos. Non-looping is defined by the failure of the ventricle to align perpendicular to the atrium. (B) Quantification of the cardiac phenotypes in wildtype embryos (wt, n=54), embryos injected with standard control MO (control, n=46), hspb7 MO (hspb7, n=67), or hspb12 MO (hspb12, n=89). (C) Quantification of the cardiac phenotypes in wildtype embryos (wt, n=20) or embryos injected with sub-threshold concentrations of MO specific for gata4 (n=38), hspb7 (n=36), or hspb12 (n=29), or with the same concentrations combined (gata4+hspb7, n=29) or (gata4+hspb12, n=49).

Expression Data

Gene:	EGFP
Fish:	twu34Tg twu34Tg + MO1-hspb7 twu34Tg + MO1-hspb12
Knockdown Reagents:	MO1-hspb7 MO1-hspb12
Anatomical Term:	heart
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	twu34Tg + MO1-hspb7 twu34Tg + MO1-hspb12
Knockdown Reagents:	MO1-hspb7 MO1-hspb12
Observed In:	heart heart looping
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 381(2), Rosenfeld, G.E., Mercer, E.J., Mason, C.E., and Evans, T., Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis, 389-400, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.