Fig. S2

grk5l and raptor Are Similarly Spatially Expressed, Related to Figures 3 and 4

Expression analysis by in situ hybridization reveals an overlapping pattern for grk5l and raptor during early development.

(A) grk5l is already maternally supplied. Picture shows expression at the 4 cell stage.

(B) Maternal expression of grk5l persists at the 16 cell stage.

(C) grk5l expression at the 256 cell stage. Animal view.

(D) At 10 hpf, grk5l is expressed in the tailbud region. Dorsal view.

(E) Flatmount of 4 ss embryo showing stronger grk5l expression in the tailbud region. Anterior to the left.

(F) Closeup of tailbud expression (4 ss).

(G) During somitogenesis, grk5l is ubiquitously transcribed at low levels. However, stronger expression can be detected in the tailbud region (12 ss).

(H) grk5l is enriched in the otic placode.

(I) At 24 hpf, grk5l is most highly expressed in the head.

(J) From 2 dpf on, grk5l expression becomes more restricted and can also be detected in the pronephros.

(K) Higher magnification of the tail of a 2 dpf embryo highlighting the expression of grk5l in the neural tube, the notochord and the pronephros.

(L) Weak, but ubiquitous expression of raptor at the tailbud stage. Lateral view, dorsal to the right.

(M) Spatially unrestricted expression of raptor during somitogenesis (8 ss).

(N) At 24 hpf, raptor is most highly expressed in the head and the tail.

(O) Overlapping with grk5l, raptor transcripts can be detected in the head and also weakly in the tail of 2 dpf embryos.

(P) Higher magnification of the tail of a 2 dpf embryo highlighting the expression of raptor. Although the expression appears to be generally lower than grk5l, it is similar in the neural tube, the notochord and the pronephros.