Fig. S2

Expression analysis of Jak1/Stat3 pathway members. (A) In situ hybridization indicating induction of jak1 and stat3 at 1 and 7 dpa with similar dynamics to il6st and socs3b. (Scale bar, 50 μm.) (B) Uninjured and 1-dpa cardiomyocyte RNAs from cmlc2:TRAP fish were processed for qPCR. il11r-α was the most abundantly expressed IL-6 family cytokine receptor in both uninjured and 1-dpa samples. Data are mean ± SEM n = 3. Expression levels were normalized to that of β-actin2, and further normalized to that of uninjured il6r-α. (C and D) qPCR indicating that cntf1 expression levels were decreased and cntf2 expression levels not significantly changed after injury. Data are mean ± SEM n = 3, *P < 0.05, Student t test (unpaired, two-tailed). Expression levels were normalized to that of β-actin2, and further normalized to that of the uninjured sample. (E) qPCR indicating relative expression levels of IL6 family ligands, indicating that il11a is the most abundantly expressed ligand at 1 dpa. Data are mean ± SEM n = 3. Expression levels were normalized to that of β-actin2, and further normalized to that of il6. (F) Sections from 1-dpa fli1:EGFP ventricles were stained with DAPI and imaged for green fluorescence. Then, the same sections were processed for in situ hybridization with an il11a probe. The il11a signal was enriched in and near fli1:EGFP⁺ nuclei. Arrowheads indicate colocalization between il11a and fli1:EGFP. (Scale bar, 50 μm.)