Fig. 9
Eye movement in 5 dpf morphant significant reduced during rotation. Both frequency and angle of rotation decline compared with control and morpahant. (A) Schematic representation of the method used to obtain eye movement recordings from embedded larvae with 3% methylcellulose. Calculate with the angle ¸ to plane of eyeball and body axis. (B) Statistics analysis for eye rotation frequency during 120 seconds. (C) Statistics anaylsis for average of eye movement angle. Left: left eyeball, Right: right eyeball. The bars represent changes in different treatment of 5 dpf larvae in three independent experiments. n=7 for each treated larvae. Results were expressed as mean±SD. **p<0.01, ***p<0.001 compared to control larvae. |
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Stage: | Day 5 |