FIGURE

Fig. 9

ID

ZDB-FIG-130906-31

Publication

Sorrell et al., 2013 - Tcf7l1 proteins cell autonomously restrict cardiomyocyte and promote endothelial specification in zebrafish

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Fig. 9

Tcf7l1s cell autonomously repress anterior endothelial cell development. ((A)and (B)) Control and Tcf7l1 deficient Tg(kdrl:EGFP) embryos. Tcf7l1 deficient Tg(kdrl:EGFP) embryos have a dramatic loss of the ACEs at 28 hpf. (C) Schematic of the cell transplantation strategy used for assessing cell autonomy. Red indicates injection with the rhodamine-dextran lineage tracer. (D) Representative image of cranial ECs from donor WT/Tg(kdrl:EGFP) cells transplanted into a WT host. View is fronto-lateral. (E) Representative image of cranial ECs from donor Tcf7l1 deficient/Tg(kdrl:EGFP) cells transplanted into a WT host. View is frontal. (F) Frequency of ACEs found in transplantation experiments. Asterisk indicates a statistically significant difference compared to control transplant experiments.

Expression Data

Gene:	EGFP
Fish:	s843Tg s843Tg + MO1-tcf7l1a + MO1-tcf7l1b + MO4-tp53
Knockdown Reagents:	MO1-tcf7l1a MO1-tcf7l1b MO4-tp53
Anatomical Term:	cranial vasculature
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s843Tg + MO1-tcf7l1a + MO1-tcf7l1b + MO4-tp53
Knockdown Reagents:	MO1-tcf7l1a MO1-tcf7l1b MO4-tp53
Observed In:	cranial vasculature anterior region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 380(2), Sorrell, M.R., Dohn, T.E., D'Aniello, E., and Waxman, J.S., Tcf7l1 proteins cell autonomously restrict cardiomyocyte and promote endothelial specification in zebrafish, 199-210, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.