FIGURE

Fig. 4

ID

ZDB-FIG-130822-38

Publication

Sah et al., 2013 - Ion channel-kinase TRPM7 is required for maintaining cardiac automaticity

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Fig. 4

TRPM7 is highly expressed in murine SAN. (A) Confocal images of an isolated SAN freshly dissociated from adult αMHC-Cre-ROSA26^mTmG heart: differential interference contrast (Left) and fluorescence GFP image (Right). (Scale bar, 50 μm.) (B) PCR across exon 17 from genomic DNA isolated from dissected KO^αMHC-Cre and WT SAN. Tail DNA from Trpm7^fl/- (-) serves as a positive control for Trpm7 exon 17 deletion. Black arrow, full-length exon 17. Red arrow, deleted exon 17. (C) Representative TRPM7 current-voltage traces measured in WT SAN, WT AVM, and KO^αMHC-Cre SAN. (D) Mean TRPM7 current density in WT SAN (I_Trpm7, _WT _SAN = 32.0 ± 6.2 pA/pF, n = 6), WT AVM (I_Trpm7, _AVM = 8.3 ± 0.9 pA/pF, n = 5), and SAN (I_Trpm7, _KOαMHC-Cre _SAN = 0.4 ± 0.2 pA/pF, n = 4, P < 0.01).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA