FIGURE

Fig. 7

ID

ZDB-FIG-130624-21

Publication

Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish

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Fig. 7

Notch signaling was not activated in the AVC of sco^te382 mutant hearts. (A–F) Tg(Tp1bglob:hmgb1-mCherry)^jh11, called Tg(Tp1:mCherry), transgenic zebrafish (Parsons et al., 2009) were bred with sco^te382/te382; Tg(kdrl:eGFP) transgenic zebrafish to generate sco^te382/+; Tg(Tp1:mCherry)/+; Tg(kdrl:eGFP)/+ zebrafish. Images were taken with agarose-embedded embryos using Zeiss 700 upright confocal microscope. Endocardial cells labeled by Tg(kdrl:eGFP) were used to project the heart shapes. Note that Tg(Tp1:mCherry) were gradually restricted in the AVC of wild-type hearts at 48hpf (A), 60hpf (C) to 72hpf (E), but were ectopically expressed in the ventricle of sco^te382 mutant hearts at 48hpf (B), 60hpf (D) and 72hpf (F). a, atrium; v, ventricle. Red arrowheads point to the AVC.

Expression Data

Genes:	GFP mCherry
Fish:	jh11Tg; la116Tg fbn2b^{te382a/te382a}; pku300^{te382b/te382b}; jh11Tg; la116Tg
Anatomical Terms:	atrioventricular canal endocardium cardiac ventricle endocardium ventricular endocardium
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fbn2b^{te382a/te382a}; pku300^{te382b/te382b}; jh11Tg; la116Tg
Observed In:	atrioventricular canal Notch signaling involved in heart development
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.