FIGURE

Fig. 5

ID

ZDB-FIG-130516-32

Publication

Schuler et al., 2013 - Ciliated sensory hair cell formation and function require the F-BAR protein syndapin I and the WH2 domain-based actin nucleator Cobl

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Fig. 5

Sensory hair cells within neuromasts of both cobl- and syndapin I-morphant zebrafish larvae are not functional. (A,D,G,J) Confocal images of representative posterior lateral line neuromasts of wild-type, p53-, sdpI- and cobl-morphant transgenic claudinB:lyn(GFP) larvae at 4dpf. Hair cells are organized symmetrically in rosettes in wild-type (A), control-injected (D), syndapin I (G) and cobl morphants (J). (B,E,H,K) FM 4-64FX vital dye labeling shows a lack of dye uptake in syndapin I (H) and cobl (K) morphants in comparison with wild-type (B) and control-injected larvae (E). (C,F,I,L) Merged images of the mostly apical FM-labeling of vesicular structures and claudin-GFP. Scale bar: 20 μm.

Expression Data

Gene:	EGFP
Fish:	zf106Tg zf106Tg + MO2-pacsin1b zf106Tg + MO3-cobl zf106Tg + MO4-tp53
Knockdown Reagents:	MO2-pacsin1b MO3-cobl MO4-tp53
Anatomical Term:	neuromast
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.