FIGURE

Fig. 8

ID

ZDB-FIG-130516-35

Publication

Schuler et al., 2013 - Ciliated sensory hair cell formation and function require the F-BAR protein syndapin I and the WH2 domain-based actin nucleator Cobl

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Fig. 8

Syndapin I and Cobl localize to the base of forming cilia. (A–M) Localization of GFP (A), GFP-syndapin I (D) and GFP-Cobl_N (G), as well as of a combination of mCherry-syndapin I (J) and GFP-Cobl (K) in ZF4 cells in relation to cilia marked by anti-acetylated-tubulin antibodies (B,E,H,L). In merged images, co-localizations appear yellow in (C,F,I) and white in (M). Scale bars: 5μm. (N) RT-PCR analysis of ZF4 cell RNA for syndapin I and cobl. DNA plasmids were used as positive controls (+control) and ef1alpha served as a technical control. Both syndapin I and cobl (marked) were detected. (O) Affinity-purified anti-syndapin I antibodies specifically recognize zebrafish GFP-syndapin I overexpressed in HEK293 cells in immunoblotting analyses. (P–R) Acetylated tubulin (P) and endogenous syndapin I (Q) in ZF4 cells. The merged image (R) additionally includes phalloidin (purple) and DAPI (blue) labeling. Insets, magnifications of boxed areas. Scale bar: 20 μm.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.