FIGURE

Fig. 2

ID

ZDB-FIG-130516-15

Publication

O'Neill et al., 2013 - BMP signaling and spadetail regulate exit of muscle precursors from the zebrafish tailbud

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Fig. 2

Chd;spt mutants produce mesodermal progenitor cells in the tailbud but are not able to form somites. (A)–(D) Dorsal view of myoD expression in 24 hpf embryos. (A)–(C) WT, chd, and spt embryos form organized somites even if it is only in the tail (spt). chd;spt mutants do not form organized somites. (60/65 have no organized somites. The remaining 5 only had 2–3 somites form.) (D). (E)–(H), dorsal view of ntn1b expressing muscle cells and tbx6 expressing MPCs in 11 hpf embryos. (E)–(G) WT, chd, and spt embryos have MPCs in tailbud as well as differentiated muscle cells outside the tailbud. chd;spt embryos have an accumulation of MPCs in the tailbud and no differentiated muscle cells. (I)–(L), lateral view of papc expression in 24 hpf embryos. WT and mutant embryos have MPCs in the tailbud with spt and chd;spt embryos having a large accumulation of progenitor cells.

Expression Data

Genes:	myod1 ntn1b pcdh8 tbx6
Fish:	WT chrd^ks1/ks1 tbx16^b104/b104 tbx16^b104/b104; chrd^ks1/ks1
Anatomical Terms:	myotome tail bud myoblast trunk muscle cell
Stage Range:	1-4 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	chrd^ks1/ks1 tbx16^b104/b104 tbx16^b104/b104; chrd^ks1/ks1
Observed In:	post-vent region somite tail bud trunk somite whole organism somite
Stage Range:	1-4 somites to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 375(2), O'Neill, K., and Thorpe, C., BMP signaling and spadetail regulate exit of muscle precursors from the zebrafish tailbud, 117-127, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.