FIGURE

Fig. 1

ID

ZDB-FIG-130429-35

Publication

Wolf et al., 2013 - Specification of posterior hypothalamic neurons requires coordinated activities of Fezf2, Otp, Sim1a and Foxb1.2

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Fig. 1

Otp regulates the expression of fezf2 and foxb1.2 in the putative mammillary area. Whole-mount in situ hybridization (WISH) to detect the expression of fezf2 and foxb1.2 in 2-dpf wild-type (A,C,E) and otpa^m866 mutant zebrafish embryos injected with 4 ng otpb morpholino (MO) (B,D,F). (A-D) In embryos lacking Otp activity, the fezf2 expression domain is strongly reduced in the PO (arrows) and expanded in the posterior part of the hypothalamus (brackets; C and D are more dorsal planes to A and B in the same embryo; n=11/11). (E,F) In embryos lacking Otp activity, foxb1.2 expression is expanded in the putative mammillary area (MA) (arrowheads; n=17/17). Dorsal views are shown. PO, preoptic area; Hyp, hypothalamus; LOF, loss-of-function. Scale bar: 100 μm.

Expression Data

Genes:	fezf2 foxb1a
Fish:	WT otpa^m866/m866 + MO3-otpb
Knockdown Reagent:	MO3-otpb
Anatomical Terms:	hypothalamus posterior region preoptic area
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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