FIGURE

Fig. S5

ID

ZDB-FIG-130326-40

Publication

Djiotsa et al., 2012 - Pax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates alpha-cell generation by repressing arx gene expression

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Fig. S5

Expression of the Tg(ARX enhancer:GFP) transgene is increased by arx knock-down. Representative images of Tg(ARX_enhancer:GFP) transgenic embryos that have been injected with arx morpholino in comparison to uninjected embryos and embryos injected with a standard control morpholino. Fluorescent overview images were taken with an inverted microscope (A), while fluorescent intensities were measured by confocal microscopy (B) and quantified (C) as described in Materials and Method. A. Lateral view of 2 dpf Tg(ARX_enhancer:GFP) embryos injected with ARX morpholino (2ng), control morpholino (2ng) or uninjected. B. Maximum intensity projection of confocal stack of 3 dpf Tg(ARX_enhancer:GFP) embryos (dorsal view) injected with ARX morpholino (2ng), control morpholino (2ng) or uninjected. C. Fluorescent intensity measurements. Means of 10 embryos ± Standard Deviation. Different from control at *P < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.