FIGURE

Fig. 1

ID

ZDB-FIG-130211-34

Publication

Ma et al., 2012 - Foxn1 maintains thymic epithelial cells to support T-cell development via mcm2 in zebrafish

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Fig. 1

T-cell development is impaired in zebrafish foxn1 morphants. (A and B) The endogenous expression level of foxn1 transcript and the encoded protein in zebrafish foxn1 morphants at 4 dpf detected by WISH (A) and Western blot (B). Ba, Western blot; Bb, Western blot results were quantified by using Quantity 1 software. (C) The rag2:dsRed expression was abolished in foxn1 morphants at 5 dpf. (D) The expression of lymphocyte markers, including rag1, il7r, and ikaros, was down-regulated in the thymus in zebrafish foxn1 morphants. Anterior to the left and dorsal to the top; circles mark the thymus. (E) qRT-PCR results showing that the expression of rag1, il7r, and ikaros is down-regulated in zebrafish foxn1 morphants (mean ± SD, t test, **P < 0.01, n = 3).

Expression Data

Genes:	DsRed foxn1 ikzf1 il7r rag1
Fish:	WT WT + MO3-foxn1 zf411Tg zf411Tg + MO3-foxn1
Knockdown Reagent:	MO3-foxn1
Anatomical Terms:	T cell thymus thymus epithelial cell
Stage Range:	Day 4 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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