FIGURE

Fig. 5

ID

ZDB-FIG-130211-38

Publication

Ma et al., 2012 - Foxn1 maintains thymic epithelial cells to support T-cell development via mcm2 in zebrafish

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Fig. 5

mcm2 is a direct target of Foxn1. (A) Predicted Foxn1 binding site and ChIP-PCR analysis of the Foxn1 binding to the promoter region of mcm2. The consensus site was marked by colored letters. (B) qRT-PCR analysis of Foxn1 binding to the promoter region of mcm2 in control and foxn1 morphants (mean ± SD, n = 3). (C) The mcm2 promoter and the mcm2p truncated constructs, and the luciferase reporter assay (mean± SD, n = 3). HEK293 cells were cotransfected by the Renilla reporter plasmid and the mcm2 promoter construct together with pCDNA3.3(+)-foxn1. Luciferase assays were determined by using the Dual-Luciferase Reporter Assay System (Promega). 2F/2R stand for a pair of gene specific primers spanning the Foxn1 binding site; CF/CR stand for control primers. The results indicate that the functional consensus Fox binding sites of the mcm2 promoter are positively regulated by Foxn1 in a dose-dependent manner.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA