FIGURE

Fig. 5

ID

ZDB-FIG-130128-45

Publication

Garnaas et al., 2012 - Rargb regulates organ laterality in a zebrafish model of right atrial isomerism

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Fig. 5

Midline hearts and bilateral livers correlate in rargb morphants. (A–F) Control embryos develop leftward jogging hearts as shown by the pan-cardiomyocyte marker cmlc2 at 26 hpf (A) and chamber-specific markers vmhc and amhc at 30 hpf (B and C), whereas rargb morphants develop midline ventricles (D and E). (G and H) Embryos treated with the RARγ antagonist MM11253 develop midline hearts, confirming genetic knockdown data. (I) RARγ function is evolutionarily conserved. The midline heart phenotype of rargb morphant embryos is rescued by injection of mouse Rarg mRNA. (J) Midline hearts and bilateral livers correlate in the same embryo. Cmlc2:GFP reporter fish injected with rargb MO were sorted by heart phenotype at 30 hpf, then liver laterality was assessed at 72 hpf by lfabp expression. Scale bars: 100 µm.

Expression Data

Genes:	myh6 myh7 myl7
Fish:	WT WT + MO1-rargb
Knockdown Reagent:	MO1-rargb
Anatomical Terms:	cardiac muscle cell presumptive atrium heart tube presumptive cardiac ventricle heart tube
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-rargb
Knockdown Reagent:	MO1-rargb
Observed In:	determination of heart left/right asymmetry presumptive cardiac ventricle heart tube
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 372(2), Garnaas, M.K., Cutting, C.C., Meyers, A., Kelsey, P.B., Harris, J.M., North, T.E., and Goessling, W., Rargb regulates organ laterality in a zebrafish model of right atrial isomerism, 178-189, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.