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Rargb knockdown impacts the left–right asymmetry of visceral organs. (A and B) Prox1 expression demonstrates that hepatic progenitors normally migrate to the left of the midline at 30 hpf (A, black arrowhead). Loss of rargb results in bilateral (B) or midline (B2) hepatoblast populations. (C-D) In situ hybridization for the pan-endodermal marker foxA3 at 48 hpf demonstrates that loss of rargb results in bilateral livers (black arrowheads) that are sometimes accompanied by bilateral pancreata (white arrowheads). (E and F) Rargb morphants develop gut looping abnormalities (arrow), as demonstrated by shh expression at 48 hpf (E and F). (G and H) Hand2 expression at 48 hpf demonstrates aberrant LPM migration in rargb morphants (H). Insets in (G and H) highlight the differences in LPM phenotypes. Scale bars: 100 ¼m.
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