ZFIN Image: Garnaas et al., 2012, Fig. 5

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Figure Caption

Fig. 5

Midline hearts and bilateral livers correlate in rargb morphants. (A–F) Control embryos develop leftward jogging hearts as shown by the pan-cardiomyocyte marker cmlc2 at 26 hpf (A) and chamber-specific markers vmhc and amhc at 30 hpf (B and C), whereas rargb morphants develop midline ventricles (D and E). (G and H) Embryos treated with the RARγ antagonist MM11253 develop midline hearts, confirming genetic knockdown data. (I) RARγ function is evolutionarily conserved. The midline heart phenotype of rargb morphant embryos is rescued by injection of mouse Rarg mRNA. (J) Midline hearts and bilateral livers correlate in the same embryo. Cmlc2:GFP reporter fish injected with rargb MO were sorted by heart phenotype at 30 hpf, then liver laterality was assessed at 72 hpf by lfabp expression. Scale bars: 100 µm.

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Acknowledgments

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Reprinted from Developmental Biology, 372(2), Garnaas, M.K., Cutting, C.C., Meyers, A., Kelsey, P.B., Harris, J.M., North, T.E., and Goessling, W., Rargb regulates organ laterality in a zebrafish model of right atrial isomerism, 178-189, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.