FIGURE

Fig. 3

ID

ZDB-FIG-130123-22

Publication

Bosco et al., 2013 - Development of hypothalamic serotoninergic neurons requires Fgf signalling via the ETS-domain transcription factor Etv5b

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Fig. 3

etv5b loss-of-function does not affect the number of cells in other basal forebrain neuron populations. (A-E) Confocal maximum intensity projections showing dopaminergic (DA) neuron clusters (4/5/6 and 7) of the basal forebrain revealed by TH1 immunohistochemistry in controls and etv5b morphants. Ventral views, anterior left. (F,G) The number of TH1-immunoreactive cells in the different DA clusters at 72 hpf in controls and etv5b ATG (0.075 mM, F) or splice (0.15 mM, G) morphants expressed as percentage of control. (H-V) Expression of oxtl, avpl and sst3 in controls and etv5b morphants. Dorsal views, anterior left. Brackets in H, M and R indicate the population included in the analysis. (W,X) The number of cells expressing oxtl, avpl or sst3 at 72 hpf in controls and etv5b ATG (0.075 mM, W) or splice (0.15 mM, X) morphants expressed as percentage of control. n, total number of embryos analysed for each experiment. n.s., not significant. Scale bars: 50 μm.

Expression Data

Genes:	avp oxt sst7
Antibody:	Ab1-serotonin
Fish:	WT WT + MO2-etv5b WT + MO3-etv5b
Knockdown Reagents:	MO2-etv5b MO3-etv5b
Anatomical Terms:	dopaminergic neuron hypothalamus neuroendocrine cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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