Fig. S1

Comparison of reporter activity in Tcf/Lef-miniP:dGFP- and TOPdGFP transgenic zebrafish embryos (A, B) Comparison of the reporter fluorescence activity in Tcf/Lef-miniP:dGFP and TOPdGFP transgenic zebrafish embryos. (A) Left side (panels for 8, 10.5, 12, and 18 hpf, and upper panels for 25, 30, and 50 hpf) and dorsal (lower panels for 25, 30, and 50 hpf) views of Line-2 or TOPdGFP transgenic zebrafish embryos, with the ventral side to the left (panels for 8, 10.5, 12, and 18 hpf) or the anterior side to the left (panels for 25, 30, and 50 hpf). Scale bar, 250 μm. (B) Panels show Line-2 and TOPdGFP transgenic embryos, which were collected randomly by non-fluorescence microscopy. (A, B) d2EGFP-expressing cells were visualized by fluorescence microscopy (right panels). Bright-field (BF) images are shown in the left panels. Scale bar, 500 μm. We confirmed that the reporter fluorescence activity was uniform in each Line-2 embryo and was much stronger than that in TOPdGFP fishes throughout embryonic development. (C) Comparison of the d2EGFP mRNA expression level in Tcf/Lef-miniP:dGFP- and TOPdGFP transgenic zebrafish embryos. At the stages indicated, 20 embryos were collected and their total RNA was purified. The relative expression levels of d2EGFP mRNAs were analyzed by qPCR. The p value was calculated by Student′s t test. We confirmed that the d2EGFP mRNA expression level in Line-2 was much higher than that in TOPdGFP fishes throughout embryonic development.