FIGURE

Fig. 4

ID

ZDB-FIG-121029-2

Publication

Ni et al., 2012 - Conditional control of gene function by an invertible gene trap in zebrafish

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Fig. 4

Liver-specific expression of supv3l1 in supv3l1^GtT/GtT mutants rescues hepatocyte defects. (A–C) Images of Lipan (A), LiPan/supv3l1^GtT/GtT (B), LiPan/hepatocyte-Cre^+/-/supv3l1^GtT/GtT (C) showing liver agenesis in supv3l1^GtT/GtT and liver-specific rescue by hepatocyte-Cre. Other defects in supv3l1^GtT/GtT remain in hepatocyte-Cre^+/-/supv3l1^GtT/GtT. Each image is a merge of a bright-field image and a red fluorescent image of the same larva. (D–F) In situ hybridization analysis of transferrin a mRNA expression in 5-dpf larvae showing the specific expression in the liver of wild-type and heterozygous larva (90/114) (D), the absence in supv3l1^GtT/GtT (12/114) (E), and the restoration in the liver-specific rescue hepatocyte-Cre^+/-/supv3l1^GtT/GtT (12/114) (F). Eye size in E and F remains smaller than in D. Observed numbers are not different from expectation (χ² test, P = 0.623). (G and H) Liver H&E staining of wild-type (G), supv3l1^GtT/GtT (H), and hepatocyte-Cre^+/-/supv3l1^GtT/GtT (I) larvae at 5 dpf, indicating the poorly differentiated hepatocytes with vacuolated cytoplasm and weak nuclear staining in global mutants (H) is fully restored by hepatocyte-Cre (I). Arrowheads indicate hepatocytes.

Expression Data

Gene:	DsRed
Fish:	gz15Tg
Anatomical Term:	liver
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	supv3l1^{vu497Gt/vu497Gt}
Observed In:	liver liver development
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA