Fig. 4

Fig. 4 Liver-specific expression of supv3l1 in supv3l1^GtT/GtT mutants rescues hepatocyte defects. (A–C) Images of Lipan (A), LiPan/supv3l1^GtT/GtT (B), LiPan/hepatocyte-Cre^+/-/supv3l1^GtT/GtT (C) showing liver agenesis in supv3l1^GtT/GtT and liver-specific rescue by hepatocyte-Cre. Other defects in supv3l1^GtT/GtT remain in hepatocyte-Cre^+/-/supv3l1^GtT/GtT. Each image is a merge of a bright-field image and a red fluorescent image of the same larva. (D–F) In situ hybridization analysis of transferrin a mRNA expression in 5-dpf larvae showing the specific expression in the liver of wild-type and heterozygous larva (90/114) (D), the absence in supv3l1^GtT/GtT (12/114) (E), and the restoration in the liver-specific rescue hepatocyte-Cre^+/-/supv3l1^GtT/GtT (12/114) (F). Eye size in E and F remains smaller than in D. Observed numbers are not different from expectation (χ² test, P = 0.623). (G and H) Liver H&E staining of wild-type (G), supv3l1^GtT/GtT (H), and hepatocyte-Cre^+/-/supv3l1^GtT/GtT (I) larvae at 5 dpf, indicating the poorly differentiated hepatocytes with vacuolated cytoplasm and weak nuclear staining in global mutants (H) is fully restored by hepatocyte-Cre (I). Arrowheads indicate hepatocytes.