FIGURE

Fig. 2

ID

ZDB-FIG-120823-5

Publication

Kim et al., 2012 - Context-dependent proangiogenic function of bone morphogenetic protein signaling is mediated by disabled homolog 2

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Fig. 2

Dab2 Functionally Interacts with Bmp2b Signal to Promote Caudal Vein Plexus Formation (A) Fluorescent images of 32 hpf control, dab2, bmpr2a, and dab2/bmpr2a MO-injected embryos. Arrowheads point to the regions that fail to connect to neighboring sprouts. Scale bar is 100 μm. See also Figure S2A.(B) Quantification of ventral vein (VV) defects. n = 3, and total number of embryos was 54 (control), 32 (bmpr2a MO), 26 (dab2 MO), or 35 (dab2/bmpr2a MO). p < 0.001 in all cases, except p value against dab2 MO-injected embryos, where p = 0.05. comparison with control MO-injected embryos; comparison with bmpr2a MO-injected embryos; and comparison with dab2 MO-injected embryos. Error bars represent SEM.(C) Confocal micrographs of control or dab2 MO-injected 45 hpf Tg(hsp70l:bmp2b) embryos, showing p-Smad-1,5/8 (red) and endothelial cells (green). Arrowheads point to p-Smad-1,5/8 within venous endothelial cells. Scale bar is 100 μm.(D) Confocal micrographs of control or dab2 MO-injected 45 hpf Tg(BRE:GFP);Tg(fli1:DsRed) embryos. Arrowheads point to GFP expression by BRE. Scale bar is 100 μm. DA, dorsal aorta; DV, dorsal vein; VV, ventral vein.

Expression Data

Gene:	EGFP
Antibody:	Ab1-smad
Fish:	pt510Tg; um13Tg fr13Tg; y7Tg pt510Tg; um13Tg + MO1-dab2 fr13Tg; y7Tg + MO1-dab2
Knockdown Reagent:	MO1-dab2
Anatomical Term:	vein endothelial cell
Stage:	High-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s843Tg + MO1-dab2 s843Tg + MO3-bmpr2a s843Tg + MO1-dab2 + MO3-bmpr2a
Knockdown Reagents:	MO1-dab2 MO3-bmpr2a
Observed In:	caudal vein plexus sprouting angiogenesis
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 23(2), Kim, J.D., Kang, H., Larrivée, B., Lee, M.Y., Mettlen, M., Schmid, S.L., Roman, B.L., Qyang, Y., Eichmann, A., and Jin, S.W., Context-dependent proangiogenic function of bone morphogenetic protein signaling is mediated by disabled homolog 2, 441-448, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell