Fig. 1

Dab2 Mediates Venous-Specific Proangiogenic Function of Bmp2b (A) Fluorescence in situ for dab2 and immunostaining for kdrl:GFP and DAPI. Lateral view (top) and transverse section (bottom). Scale bar is 200 μm (top) or 10 μm (bottom). DA, dorsal aorta; CV, cardinal vein. See also Figure S1A.(B) Depth-coded images of control or dab2 MO-injected 45 hpf wild-type (WT) and Bmp2b overexpressing (Bmp2) embryos. XZ and YZ axis views of each image are also shown. Arrows point to ectopic vessels in the projection. Images were obtained between the 16^th and 20^th somite. Scale bar is 100 μm. DA, dorsal aorta; DV, dorsal vein; VV, ventral vein. See also Figures S1B–S1F.(C) Quantification. The number of branches (n = 3, total number of embryos was 19 [Bmp2b] or 17 [Bmp2b/dab2 MO], p < 0.001) and vessel surface as assessed by mean GFP pixel intensity (n = 3, total number of embryos was 21 [Bmp2b] or 19 [Bmp2b/dab2 MO], p < 0.001). Error bars represent standard error of the mean (SEM).(D) Fluorescent images of 26 hpf control (top) or dab2 (bottom) MO-injected embryos. Arrowheads point to ventral sprouts. Scale bar is 100 μm.(E) Quantification of ventral sprouts in the CVP (n = 3, total number of embryos was 25 [Control] or 20 [dab2 MO], p < 0.001). Error bars represent SEM.(F) Fibrin gel assay of control and DAB2 siRNA-treated HUVECs. Scale bar is 10 μm. See also Figures S1G and S1H.(G) Quantification of vascular coverage and diameter. Values were normalized to control siRNA treatment sample by PRISM program. n = 3 and p < 0.05. Error bar is SEM.